Resumo Neste artigo propomos-nos, como objetivo, fazer reflexões metodológicas sobre as nossas experiências como investigadores no campo da segurança. Explicamos alguns nós problemáticos em torno do trabalho de investigação qualitativa no contato direto com a polícia, em parte para abrir debates e discussões no campo das ciências sociais, mas também como uma reflexividade para nossas investigações em andamento. Antecipamos que o espírito do artigo tente debater alguns aspectos do “acesso” ao trabalho de campo dentro da instituição policial, ressaltando a relação entre a suposta opacidade e a predisposição de seus agentes em nos atender e responder às nossas perguntas. Para isso, abordamos alguns momentos da nossa pesquisa que nos permitem identificar e refletir sobre as nossas estratégias de construção da confiança e alguns mal-entendidos que suscitam manifestações de desconfiança sobre nossas intenções.

Abstract This article has the objective of making methodological reflections on our experiences as researchers in the field of security. We explain some problematic knots around the office of qualitatively investigating in direct contact with the police, partly to open debates and discussions within the field of social sciences, but also as a reflexivity exercise for our ongoing investigations. We anticipate that the spirit of the article attempts to resolve some aspects of “access” to field work within the police institution, stressing the relationship between the alleged opacity and the predisposition of its agents to meet us and answer our questions. To do so, we address some moments of our research that allow us to identify and reflect on our strategies to build trust and some misunderstandings that gave rise to expressions of suspicion about ourselves and our intentions.

Resumen En este artículo nos planteamos el objetivo de hacer reflexiones metodológicas sobre nuestras experiencias como investigadoras en el campo de la seguridad. Explicitamos algunos nudos problemáticos en torno al oficio de investigar cualitativamente en contacto directo con la policía, en parte para abrir debates y discusiones dentro del campo de las ciencias sociales, pero también a modo de reflexividad para nuestras indagaciones en curso. Adelantamos que el espíritu del artículo intenta dirimir algunos aspectos sobre el “acceso” al trabajo de campo dentro de la institución policial, que tensiona la relación entre la presunta opacidad y la predisposición de sus agentes para encontrarse con nosotras y responder nuestras preguntas. Para ello, abordamos algunos momentos de nuestra pesquisa que nos permiten identificar y reflexionar en torno de nuestras estrategias para construir confianza y algunos equívocos que dan lugar a expresiones de sospecha sobre nuestra persona y nuestras intenciones.

Abstract To characterize the reproductive biology of Scinax acuminatus and contribute to the natural history of this species, the morphology of the reproductive system of males and females was analyzed at anatomical, histological and immunohistochemical levels. The individuals were collected fortnightly between August and December (2016) and January to December (2018). The anatomy of the reproductive system was analyzed in a stereoscopic microscope, and histological preparations staining with hematoxylin-eosin and Masson’s trichromic, PAS and Coomassie Blue was performed as well. To characterize the meiotic-active cells in the testes, immunostaining with the PCNA proliferation protein was performed. There were found females with ovaries with oocytes in different stages of maturity and post-ovulatory females. Males presented continuous spermatogenesis, which could be confirmed by the immunostaining of PCNA in spermatogonia during the cycle. The results of this work serve as a basis for the characterization of the reproductive cycle in S. acuminatus and provide background information on the analysis of spermatogenic activity by IHQ from the study of the immunodetection of the PCNA cell proliferation protein. Future studies will focus on the evaluation of cell death processes during the reproductive cycle in the studied species to compare with those obtained in terms of cell proliferation.